منابع مشابه
Regulation of Cell-mediated Cytotoxicity
Mice were treated with sublethal and midlethal doses of irradiation (500-700 rads) and injected intravenously with allogeneic or semiallogeneic F(1) hybrid spleen cells. The cytotoxicity developed by their spleen cells was measured with the lysis of (51)Cr-labeled target cells and was found to be stronger (although delayed in time) than the cytotoxic activity of spleen cells from nonirradiated ...
متن کاملPeroxidase-mediated Mammalian Cell Cytotoxicity
Lactoperoxidase, in the presence of hydrogen peroxide and iodide is cytotoxic for human and mouse lymphoid cells, and human erythrocytes. Myeloperoxidase, in amounts equivalent to 1.5 x 10(6) neutrophils, readily replaces lactoperoxidase, and allows the substitution of the iodide ion by chloride. The myeloperoxidase-mediated reaction is rapid, and highly efficient, leading to 85-90% cell death ...
متن کاملUsing impedance-based approaches for measuring cell-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC)
The most common method for measuring cell-mediated cytotoxicity is the release assay. Effector cell-mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. Endogenous biomolecules (such as lactate dehydrogenase) or previously added exogenous labels (such as the radioisotopes Cr or In) that leak into the media are then measured as a...
متن کاملReal Time Assays for Quantifying Cytotoxicity with Single Cell Resolution
A new live cell-based assay platform has been developed for the determination of complement dependent cytotoxicity (CDC), antibody dependent cellular cytotoxicity (ADCC), and overall cytotoxicity in human whole blood. In these assays, the targeted tumor cell populations are first labeled with fluorescent Cell Tracker dyes and immobilized using a DNA-based adhesion technique. This allows the fac...
متن کاملMolecular Mechanisms in Cell-Mediated Cytotoxicity
terleukin-1b converting enzyme (ICE) family, now called Department of Biomedical Science the caspase family (Alnemri et al., 1996), is believed to and Biotechnologies be required for cell death, at least as to its nuclear University of Brescia manifestations. GraB can cleave and thus activate cas25100 Brescia pases, leading tocell death. This pathway was examined and Institute of Histology by C...
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ژورنال
عنوان ژورنال: Asthma Allergy Immunology
سال: 2019
ISSN: 1308-9234
DOI: 10.21911/aai.403